Genome-wide reconstitution of chromatin transactions reveals that RSC preferentially disrupts H2AZ-containing nucleosomes
نویسندگان
چکیده
منابع مشابه
RSC mobilizes nucleosomes to improve accessibility of repair machinery to the damaged chromatin.
Repair of DNA double-strand breaks (DSBs) protects cells and organisms, as well as their genome integrity. Since DSB repair occurs in the context of chromatin, chromatin must be modified to prevent it from inhibiting DSB repair. Evidence supports the role of histone modifications and ATP-dependent chromatin remodeling in repair and signaling of chromosome DSBs. The key questions are, then, what...
متن کاملThe RSC chromatin remodelling enzyme has a unique role in directing the accurate positioning of nucleosomes.
Nucleosomes impede access to DNA. Therefore, nucleosome positioning is fundamental to genome regulation. Nevertheless, the molecular nucleosome positioning mechanisms are poorly understood. This is partly because in vitro reconstitution of in vivo-like nucleosome positions from purified components is mostly lacking, barring biochemical studies. Using a yeast extract in vitro reconstitution syst...
متن کاملThe RSC chromatin remodeling enzyme plays a unique role in directing the accurate positioning of nucleosomes
Thank you for submitting your manuscript for consideration at The EMBO Journal. I apologise that it has taken so long to evaluate your manuscript, but I understand that my colleague forwarded you the reports while I was away. Overall the three referees that evaluated your study find it to be interesting, however, there are a number of concerns and issues that need to be addressed. One of the ma...
متن کاملGenome-Wide Association between Transcription Factor Expression and Chromatin Accessibility Reveals Regulators of Chromatin Accessibility
To better understand genome regulation, it is important to uncover the role of transcription factors in the process of chromatin structure establishment and maintenance. Here we present a data-driven approach to systematically characterise transcription factors that are relevant for this process. Our method uses a linear mixed modelling approach to combine datasets of transcription factor bindi...
متن کاملThe genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress.
The cellular response to environmental changes includes widespread modifications in gene expression. Here we report the identification and characterization of Rsc9, a member of the RSC chromatin-remodeling complex in yeast. The genome-wide localization of Rsc9 indicated a relationship between genes targeted by Rsc9 and genes regulated by stress; treatment with hydrogen peroxide or rapamycin, wh...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Research
سال: 2019
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.243139.118